Primer
Part:BBa_K268025:Design
Designed by: Nora Yucel Group: iGEM09_UChicago (2009-10-21)
YGR243C -> GFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2461
Illegal PstI site found at 1225 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2461
Illegal PstI site found at 1225 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2461
Illegal BglII site found at 1015
Illegal BamHI site found at 44 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2461
Illegal PstI site found at 1225 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2461
Illegal PstI site found at 1225 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 704
Design Notes
Source
Promoter region from gene YGR243C in S. cerevisiae as obtained through yeastgenome.org. Gene deletion and tagging cassette from Longtine, et al (1998).